Indirect Spectrophotometric Determination of Metformin Via Decolorization Eriochrome Black-T

Abstract

The research included the description of a simple indirect spectrophotometric method for the determination of Metformin, which depended on the oxidation of Metformin with an amount of N- bromosuccinimide in the presence of acetic acid and the excess amount of it oxidized the dye Eriochrome black-T, and the absorption was measured at 520 nm, and the molar absorption coefficient was 4 × 104 L / mol. cm, indicating the sensitivity of the method. And compliance with Beer's law for concentrations ranged from (2-16) micrograms. ML-1, the method was characterized by good accuracy and compatibility, with a recoverability of 100.155%. While the relative standard deviation was less than 1.0.The method was applied to
pharmaceutical preparations of Metformin hydrochloride.