Spectrophotometric determination of Hydrochlorothiazide by diazotization- coupling using a meta-aminophenol reagent and application to pharmaceutical formulations.

Abstract

A Spectrophotometry method was developed to be faster and more sensitive to determine the purity of Hydrochlorothiazide compound. This method is relying on the reaction of diazotization- coupling using a meta-aminophenol reagent medium and factor to give a yellow product at the wavelength 443 nm. This is will be within the range of Beer's law (0.4-20 ppm), molar absorbance (3.7843×10⁴ L.mol^-1.cm^-1), correlation coefficient (0.9998), and Sandell?s sensitivity (0.00786 µg/cm²) Limit of detection (LOD) (0.0226 ppm) and LOQ (0.0754 ppm). It has been successfully applied to pharmaceutical preparations...