Association of H. pylori IgG and CagA-IgG with some immunological and biochemical parameters in diabetic patients.

Abstract

Helicobacter pylori a gram-negative, microaerophilic, spiral-shaped, and flagellated bacteria whose primary reservoir is the human stomach. It can cause gastric and extra-gastric diseases. So the study aimed to find the relationship between infection with H. pylori and progression of type II diabetes with other immunological markers. Method This study was conducted in Kirkuk City from Nov. 2022 to Apr. 2023 and included 106 individuals, 26 as a control group and 80 patients (34 males and 46 females) with mean age 57.61 suffering from DMII with H. pylori stool antigen positive results (immunochromatographic test cassette). Five ml of venous blood samples were taken for the HbA1c test (immunodetection technique), fast serum glucose (Hexokinase/G-6-PDH), serum H. pylori IgG, H. pylori CagA IgG, Interlukin-1?, and Interleukin-10 analysis (Sandwich-ELISA technique). Results There was a positive correlation between H. pylori IgG titer and HbA1c levels (0.0321, P = 0.004). In addition, there was a positive correlation between the levels of IL-1? and the H. pylori CagA IgG (r =0.579, P = 0.000). While a negative correlation was found between HbA1c level and IL-10 concentration, (r = -0.500, P = 0.000), there was also a negative association between IL-1? and IL-10 concentrations (r = -0.485, P = 0.000). Conclusion: Through the results of this correlation, we suggest a relationship between H. pylori infection and DMII.