Characterization of the Root-Knot Nematodes Infecting Olive Trees
DOI:
https://doi.org/10.56286/dghnfc02Keywords:
Nematoda Meloidogyne javanica Olive trees ParasiteAbstract
This study was conducted in 2022–2023 to identify the plant parasitic nematodes that infect olive trees. A field survey was conducted in the most olive-growing areas in Bashiqa and Al-Fadhiliya, Nineveh Governorate, Iraq. The results recorded the parasitism of the nematode Meloidogyne javanica on the olive roots. The formation of root knots on the roots was the main disease symptom. The phenotypic characteristics and perineal pattern of adult females indicated M. javanica, which was used to identify the species. DNA barcoding of the 18SrDNA gene with the primer sets 18S-530R and 18S-CL-F3 yielded a 494-bp fragment to identify M. javanica. The sequence blasted with the GenBank database gave a maximum similarity of 97% to the global isolates. The sequence was submitted and deposited with the NCBI as an Iraqi isolate under accession number PP273501. This is considered the first record of M. javanica on olive trees in Nineveh Governorate.
References
Joshi, I., Kumar, A., Singh, A. K., Kohli, D., Raman, K. V., Sirohi, A., & Jain, P. K. (2019). Development of nematode resistance in Arabidopsis by HD-RNAi-mediated silencing of the effector gene Mi-msp2. Scientific Reports, 9(1), 17404 https://doi.org/10.1038/s41598-019-53485-8
Álvarez-Ortega, S., Brito, J. A., & Subbotin, S. A. (2019). Multigene phylogeny of root-knot nematodes and molecular characterization of Meloidogyne nataliei Golden, Rose & Bird, 1981 (Nematoda: Tylenchida). Scientific reports, 9(1), 11788.? https://doi.org/10.1038/s41598-019-48195-0
Vélez Zambrano, S. M., & Guzmán Cedeño, A. M. (2022). Técnicas de identificación del nematodo agallador Meloidogyne. Manglar, 19(2), 209-215.? http://dx.doi.org/10.17268/manglar.2022.026
Aït Hamza, M., Ali, N., Tavoillot, J., Fossati-Gaschignard, O., Boubaker, H., El Mousadik, A., & Mateille, T. (2017). Diversity of root-knot nematodes in Moroccan olive nurseries and orchards: does Meloidogyne javanica disperse according to invasion processes. BMC ecology, 17, 1-13. https://doi.org/10.1186/s12898-017-0153-9
Abu Gharbieh, W. I; A. S. Al-Hazmi, Z. A. Stephan and A.A. Duaaba (2010) Plant Nematology in the Arab Countries (Part 1). First edition.
Ali, N., Tavoillot, J., Chapuis, E., & Mateille, T. (2016). Trend to explain the distribution of root-knot nematodes Meloidogyne spp. associated with olive trees in Morocco. Agriculture, Ecosystems & Environment, 225, 22-32.? https://doi.org/10.1016/j.agee.2016.03.042
Hanoon, W. M., and Altememe, Z. A. (2018). Morphological and Molecular Diagnosis of Root Knot Nematode Species Associated with Olive Seedling and Trees in Baghdad, Babil and Karbala/Iraq. Rafidain Journal of Science,27(4), 138-150.
Al-Hakeem, A. M., Kandouh, B. H., & Aljuboori, F. K. (2020). Plant parasitic nematodes in Iraq: occurrence and distribution records. International Journal of Agricultural & Statistical Sciences, 16.
Cunha, T. G. D., Visôtto, L. E., Lopes, E. A., Oliveira, C. M. G., & God, P. I. V. G. (2018). Diagnostic methods for identification of root-knot nematodes species from Brazil. Ciência Rural, 48, e20170449. http://dx.doi.org/10.1590/0103-8478cr20170449
Ravichandra, N. G. (2010). Methods and techniques in plant nematology. PHI Learning Pvt. Ltd.
Coyne, D. L. (2007). Practical plant nematology: a field and laboratory guide. Iita.SP-IPM Secretariat, International Institute of Tropical Agriculture (IITA), Cotonou, Benin. p1-38.
Eisenback, J. D., Hrischmann, H., Sasser, J. N., & Triantaphyllou, A. C. (1981). A guide to the four most common species of root-knot nematodes (Meloidogyne spp.), with a pictorial key (p. 48). State University, Depto. of Plant Pathology. Environ. 2016;225:22–32. https://doi.org/10.1016/j.agee.2016.03.042
Kim, H., Lee, W., & Jeong, R. (2023). Molecular Phylogeny of the Genus Paracanthonchus (Nematoda: Chromadorida) with Description of P. yeongjongensis sp. nov. from Korea. Diversity, 15(5), 664. https://doi.org/10.3390/d15050664
Hussey R. S. and Barker K. R.. 1973. Comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter 57 12: 1025–8. [Google Scholar]
Mai, W. F. (1988). Pictorial key to genera of plant-parasitic nematodes. In Nematode Identification and Expert System Technology (pp. 31-34). Springer, Boston, MA
Rusinque, L., Inácio, M. L., Mota, M., and Nóbrega, F. (2018). Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato. Revista de Ciências Agrárias, 41(1), 193-198. http://dx.doi.org/10.19084/RCA17078
Ye W, Zeng Y, Kerns J. (2015). Molecular Characterisation and Diagnosis of Root-Knot Nematodes (Meloidogyne spp.) from Turfgrasses in North Carolina, USA. PLOS ONE 10(11): e0143556. https://doi.org/10.1371/journal.pone.0143556
Assunção, M. C., & Souza Junior, F. J. C. (2022). First report of Meloidogyne javanica on Celosia argentea in the Northeast of Brazil. Diversitas Journal, 7(3). https://doi.org/10.48017/dj.v7i3.2326