Extraction of oregano oil, identification of its active compounds and its effect on the sensory evaluation of cheese
DOI:
https://doi.org/10.56286/NTU-JAVS.2026.6.1.1-6Keywords:
oregano oil, GC-Maas, CheeseAbstract
The study results showed that the percentage of oregano oil extracted by hydrodistillation technique reached 1.6%, as every 50 g. of oregano herbs gave 0.8 ml oil. The physical properties of the extracted oil were refractive index (1.338) and relative density (0.9236). The oil color was light yellow. The chemical properties of the oregano oil included a peroxide value of 7.2, and an acidity 1.683. The results of the analysis of the components of the oil in the GC-Maas device showed the identification of 30 compounds, and the carvacrol compound was the highest as its percentage reached 51.51%, which is attributed to the inhibitory effect of the oil in addition to other compounds. The evaluation results of the quality of cheese added to oregano oil at a concentration of 0.25 of the weight of the clot with cheese not treated with oil and the treated cheese were identified by smell and taste due to the clear effect of the aromatic oils. No significant differences were observed on the first day between the control treatment and the treatment containing 0.25% oil. In comparison, there were significant differences in all characteristics except the texture characteristic with the cheese containing 0.75% oil on the same day. The sensory evaluation also showed that the control cheese became unacceptable on the fourteenth day of storage at the refrigerator temperature. As for the 0.75 treatment, the results showed from the first week for the two characteristics of flavor and bitterness that they were unacceptable by the evaluation committee members, while the cheese treated with 0.25% oil showed an acceptable flavor and good taste even after twenty-one days.
References
Abelardo AGUIRRE-OROZCO, M., Lilia DELGADO-MARTÍNEZ, M., MÁRQUEZ-MONÁRREZ, O., & José CONTRERAS-MARTÍNEZ, J. (2022). Oregano oil extraction process using the" Coreganoil" coil steam drag method. Journal of Natural & Agricultural Sciences/Revista de Ciencias Naturales & Agropecuarias, 9(25).
Albado Plaus, E., Saez Flores, G., & Grabiel Ataucusi, S. (2001). Composición química y actividad antibacteriana del aceite esencial del Origanum vulgare (orégano). Revista Medica Herediana, 12(1), 16-19.
Albado Plaus, E., Saez Flores, G., & Grabiel Ataucusi, S. (2001). Composición química y actividad antibacteriana del aceite esencial del Origanum vulgare (orégano). Revista Medica Herediana, 12(1), 16-19
Al-Hijazeen, M. (2021). The combination effect of adding rosemary extract and oregano essential oil on ground chicken meat quality. Food Science and Technology, 42, e57120
Alkhafaji, R. T. H., & Jayashankar, M. (2022). Physicochemical properties and inhibitory effects of oregano oil against uropathogenic. Pharmacognosy Research, 14(3).
Asensio, C. M., Grosso, N. R., & Juliani, H. R. (2015). Quality characters, chemical composition and biological activities of oregano (Origanum spp.) Essential oils from Central and Southern Argentina. Industrial Crops and Products, 63, 203-213.
asouli, S. R., Ebrahimi, S. N., & Rezadoost, H. (2021). Simultaneous ultrasound assisted hydrodistillation of essential oil from aerial parts of the Satureja khuzistanica Jamzad and its antibacterial activity. Journal of Medicinal Plants, 20(80), 47-59.
Badawi, S. Kh , Al-Badrany Gh H. Mohammed S.. H. (2009). USING OF ESSENTIAL OILS EXTRACTED FROM SOME SPICES TO EXTEND THE SHELF LIFE OF SOFT WHITE CHEECE. Mesopotamia Journal of Agriculture, 37(4).
Boydag, I., Kurkcuoglu, M., Ozek, T., & Baser, K. H. C. (2003). Isolation of some soluble and dispersed materials of oregano water. Chemistry of natural compounds, 39, 465-469
Christaki, S., Moschakis, T., Kyriakoudi, A., Biliaderis, C. G., & Mourtzinos, I. (2021). Recent advances in plant essential oils and extracts: Delivery systems and potential uses as preservatives and antioxidants in cheese. Trends in Food Science & Technology, 116, 264-278.
Fisher, K., & Phillips, C. (2008). Potential antimicrobial uses of essential oils in food: is citrus the answer?. Trends in food science & technology, 19(3), 156-164
Fox, P. F., Guinee, T. P., Cogan, T. M., & McSweeney, P. L. (2017). Fundamentals of cheese science.
Garofalo, G., Ponte, M., Greco, C., Barbera, M., Mammano, M. M., Fascella, G., ... & Gaglio, R. (2023). Improvement of Fresh Ovine “Tuma” Cheese Quality Characteristics by Application of Oregano Essential Oils. Antioxidants, 12(6), 1293.
Giuliani, C., Maggi, F., Papa, F., & Maleci Bini, L. (2013). Congruence of phytochemical and morphological profiles along an altitudinal gradient in Origanum vulgare ssp. vulgare from Venetian region (NE Italy). Chemistry & biodiversity, 10(4), 569-583.
Guynot, M. E., Ramos, A. J., Seto, L., Purroy, P., Sanchis, V., & Marin, S. (2003). Antifungal activity of volatile compounds generated by essential oils against fungi commonly causing deterioration of bakery products. Journal of Applied
Leite, S. M. B., da Silva Assunção, E. M., Alves, A. V. D. N. G., de Souza Maciel, E., de Moraes Pinto, L. A., Kaneko, I. N., ... & Monteschio, J. D. O. (2022). Incorporation of copaiba and oregano essential oils on the shelf life of fresh ground beef patties under display: Evaluation of their impact on quality parameters and sensory attributes. Plos one, 17(8), e0272852
Leyva-López, N., Gutiérrez-Grijalva, E. P., Vazquez-Olivo, G., & Heredia, J. B. (2017). Essential oils of oregano: Biological activity beyond their antimicrobial properties. Molecules, 22(6), 989.
Medini, H., Elaissi, A., Farhat, F., Khouja, M. L., Chemli, R., & Harzallah?Skhiri, F. (2009). Seasonal and geographical influences on the chemical composition of Juniperus phoenicea L. essential oil leaves from the Northern Tunisia. Chemistry & biodiversity, 6(9), 1378-1387.
Pirigharnaei, M., Zare, S., Heidary, R., Khara, J., EmamaliSabzi, R., & Kheiry, F. (2011). The essential oils compositions of Iranian Oregano (Origanum vulgareL.) populations in field and provenance from Piranshahr district, West Azarbaijan province, Iran. Avicenna Journal of Phytomedicine, 1(2), 106-114.
Ruiz-Gonzalez, N., Lopez-Malo, A., Palou, E., Ramirez-Corona, N., & Jimenez Munguia, M. T. (2019). Antimicrobial activity and physicochemical characterization of oregano, thyme and clove leave essential oils, nonencapsulated and nanoencapsulated, using emulsification. Applied Food Biotechnology, 6(4), 237-246.
Schuh, J., Batisteli, P., Gargetti, A., Zapparoli, A., Balsan, T. I., Gilioli, A., ... & SILVEIRA, S. M. D. (2022). Basil, marjoram, nutmeg and oregano essential oils as natural preservatives of Quark-type cheese. Food Science and Technology, 42, e31322.
Shahbazi, Y., & Shavisi, N. (2019). Effects of Oregano Methanolic Extract on the Chemical, Microbial, and Sensory Properties of Yogurt. Journal of Fasting & Health, 7(3).
Solanki, J., Khanpara, P., Tilva, T., & Faldu, S. (2023). A REVIEW ON MIRACLE PLANT OREGANO. World Journal of Pharmaceutical Research, Volume 12, Issue 20, 271-303.
Tellier ,J. Johnson, ND . (2010). purely Healthful oil oregano . Canada , Mind Publishing Inc .
Xin, Y., Liu, H., Yan, X., Huang, W., Pan, S., Zhou, M., ... & Yang, Y. (2022). Effect of dietary oregano oil on growth performance, disease resistance, intestinal morphology, immunity, and microbiota of hybrid grouper (Epinephelus fuscoguttatus?× Epinephelus lanceolatus?). Frontiers in Marine Science, 9,1038394
Yuan, Y., Sun, J., Song, Y., Raka, R. N., Xiang, J., Wu, H., ... & Hui, X. (2023). Antibacterial activity of oregano essential oils against Streptococcus mutans in vitro and analysis of active components. BMC Complementary Medicine and Therapies, 23(1), 61.
Zidan, Ibrahim Al-Dawar Abdullah. (2004). Unique specifications of dairy food products between reality and expectations. Department of Dairy Science and Technology - Faculty of Agriculture - Alexandria University - Publisher Bustan Al-Maarifa Library – Alexandria



