Effect of Bio Control Agents on Controlling Root Rot and Damping-off Disease of Cucumber Seedlings Caused by Rhizoctonia solani

Authors

  • Ali Thanoon University of Mosul
  • Zainab Adel Abd Al-Qader University of Mosul

DOI:

https://doi.org/10.56286/1rk5gp32

Keywords:

cucumber, Rhizoctonia solani, root rot, Trichoderma harzianum

Abstract

Root rot and seedlings damping-off disease caused by Rhizoctonia solani is the most damaging disease of cucumber. This disease causes reducing yield in cucumber. This study conducted laboratory and greenhouse conditions to find out the efficacy of biological control agents, Trichoderma harzianum, Bacillus subtilis, the chemical compound Bion, and fungicide revanol. The results of pathogenicity test of R. solani on cucumber seeds showed that R. solanihas led to a significant reduction in the percentage of germination and seed rot compared to the control treatment, which amounted to 100%. The results showed the ability of all control agents used in this study to reduce the incidence of the disease. The Bion (benzothiadiazole) treatment showed significant effect and outperformed the other treatments in increasing the activity of the peroxidase enzyme, which was recorded at 14,440 (unit. minute-1.g wet weight-1). It also achieved the highest significant increase in total phenolics 1.52 (mg -1 . gram wet weight -1). Biological control agent T. harzianum has outperformed on other treatments in causing an increase in the activity of the polyphenol oxidase enzyme, it has recorded 1811.7 (unit. minute - 1 .gram of wet weight - 1 leaf).

References

Adams, P., Graves, C. J., & Winsor, G. W. (1992). Some responses of cucumber, grown in beds of peat, to N, K and Mg. Journal of horticultural science, 67(6), 877-884. https://doi.org/10.1080/00221589.1992.11516321.

?[2] Moataza, M. S. (2006). Destruction of Rhizoctonia solani and Phytophthora capsici causing tomato root-rot by Pseudomonas fluorescences lytic enzymes. Res J Agri Biol Sci, 2(6), 274-281.

?[3] Aljawasim, B. D. G., Khaeim, H. M., & Manshood, M. A. (2020). Assessment of biocontrol potential of arbuscular mycorrhizal (Glomus spp.) against damping-off disease (Rhizoctonia solani) on cucumber. In Mycorrhizal Fungi-Utilization in Agriculture and Forestry. IntechOpen.doi: 10.5772/intechopen.93313.

Nicolopoulou-Stamati, P., Maipas, S., Kotampasi, C., Stamatis, P., & Hens, L. (2016). Chemical pesticides and human health: the urgent need for a new concept in agriculture. Frontiers in public health, 4, 148.https://doi.org/10.3389/fpubh.2016.00148.

Afifi, M. M. I., Ismail, A. M., Kamel, S. M., & Essa, T. A. (2017). Humic substances: a powerful tool for controlling fusarium wilt disease and improving the growth of cucumber plants. Journal of Plant Pathology, 61-67.https://www.jstor.org/stable/i40179175.

Ba?maga, M., Wyszkowska, J., & Kucharski, J. (2019). The biochemical activity of soil contaminated with fungicides. Journal of Environmental Science and Health, Part B, 54(4), 252-262.https://doi.org/10.1080/03601234.2018.1553908.

Wang, Y. H., Joobeur, T., Dean, R. A., & Staub, J. E. (2007). Cucurbits-genome mapping and molecular breeding in plants 5. Nature Genetics, 41, 1275-1281.

Awad, L. K., & Fayyadh, M. A. (2018). The activity of some Actinomycetes isolates in control of cucumber damping off disease caused by Rhizoctiona solani and Pythium sp. Basrah journal of Agricultural sciences, 31(2), 11-23. https://doi.org/10.37077/25200860.2018.95.

Mahmoud, M. A. (2015). Efficiency of some bioagents and nemastop compound in controlling damping off and root rot diseases on peanut plants. Int. J. of Adv. Res. in Biological Sciences, 2(11), 77-86. http://s-o-i.org/ 1.15/ijarbs-2-11-12.

Hassan, M., Ahmed, H., Kamel, S., El-Hamed, A., & Yousef, H. (2021). Biological control of damping-off and root rot disease caused by Rhizoctonia solani on cucumber plants. Fayoum Journal of Agricultural Research and Development, 35(3), 525-541. . DOI 10.21608/FJARD.2021.225257.

Roy, S., Kumar, U., Kumari, M., Kumari, D., Yadav, M., Prasad, R., ... & Kumari, P. (2017). A review on fusarium wilt disease of chickpea and different strategies for its management. Journal of Pharmacognosy and Phytochemistry, 6(6), 2605-2613.

Ambhore, P. K., Khaire, P. B., & Desai, R. S. (2018). Efficacy of Seed Treatment with Fungicides and Bioagent on Germination and Percent Disease Incidence of Chickpea Wet Root Rot Rhizoctonia solani (Kuhn)(Sick soil/pot culture). Int. J. Curr. Microbiol. App. Sci, 7(10), 1903-1907.https://doi.org/10.20546/ijcmas.2018.710.219.

Dewan, M. M., & Sivasithamparam, K. (1988). A plant-growth-promoting sterile fungus from wheat and rye-grass roots with potential for suppressing take-all. Transactions of the British Mycological Society, 91(4), 687-692.https://doi.org/10.1016/S0007-1536(88)80045-7.

Mishra, C. B. P., & Behr, L. (1976). Der Einfluß von Kulturfiltraten von Fusarium culmorum (WG Sm.) Sacc., F. avenaceum (Fr.) Sacc. und F. nivale (Fr.) Ces.(Griphosphaeria nivalis Müller et v. Arx) auf die Keimung des Weizens. Archives of Phytopathology & Plant Protection, 12(6), 373-377.https://doi.org/10.1080/03235407609431776.

Müftügil, N. (1985). The peroxidase enzyme activity of some vegetables and its resistance to heat. Journal of the Science of Food and Agriculture, 36(9), 877-880.DOI:10.1002./JSFA.2740360918

Shi, C., Dai, Y., Xu, X., Xie, Y., & Liu, Q. (2002). The purification of polyphenol oxidase from tobacco. Protein expression and purification, 24(1), 51-55.https://doi.org/10.1006/prep.2001.1543.

Aberoumand, A., & Deokule, S. S. (2008). Comparison of phenolic compounds of some edible plants of Iran and India. Pakistan Journal of Nutrition, 7(4), 582-585.https://scialert.net/abstract/?doi=pjn.2008.582.585.

Chandra, S., Khan, S., Avula, B., Lata, H., Yang, M. H., ElSohly, M. A., & Khan, I. A. (2014). Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: A comparative study. Evidence-based complementary and alternative medicine.https://doi.org/10.1155/2014/253875.

Parmeter, J.R. and H.S. Whitney. (1970). Taxonomy and Nomenclature of the imperfect stage. Pages 7-19. In: Rhizoctonia solani biology and pathology. University of California at Berkley.Pathology, 61(1), 132-139.

Ajayi?Oyetunde, O. O., & Bradley, C. A. (2018). Rhizoctonia solani: taxonomy, population biology and management of rhizoctonia seedling disease of soybean. Plant pathology, 67(1), 3-17.https://doi.org/10.1111/ppa.12733.

Tjimune, R., Mangwende, E., Lekota, M., & Muzhinji, N. (2022). First Report of Rhizoctonia solani AG 3?PT causing black scurf on potato tubers in Namibia. New Disease Reports, 45(1).https://doi.org/10.1002/ndr2.12066.

Buzi, A., Chilosi, G., De Sillo, D., & Magro, P. (2004). Induction of resistance in melon to Didymella bryoniae and Sclerotinia sclerotiorum by seed treatments with acibenzolar?S?methyl and methyl jasmonate but not with salicylic acid. Journal of Phytopathology, 152(1), 34-42.

Chen, N., Hu, M., Qiao, C., Nai, X., & Wang, R. (2010). Effects of BTH, SA, and SiO2 treatment on disease resistance and leaf HRGP and lignin contents of melon seedlings. Scientia Agricultura Sinica, 43(3), 535-541. Sci Agric Sin 43(3):535–541, 2010.

Govindappa, M., Lokesh, S., Rai, V. R., Naik, V. R., & Raju, S. G. (2010). Induction of systemic resistance and management of safflower Macrophomina phaseolina root-rot disease by biocontrol agents. Archives of Phytopathology and Plant Protection, 43(1), 26-40.

El-Fiki, A. I. I., El-Habaa, G., Flaifel, S. M. A., Hafez, M. A., & Abdel-Ghany, R. E. A. (2007). Response of strawberry plants to some abiotic inducers under stress of some root rot pathogens. Annual Agriculture Science, Moshtohor, 45(4), 1407-1417.https://www.researchgate.net/publication/272175065.

Nafie, E., & Mazen, M. M. (2008). Chemical-induced resistance against brown stem rot in soybean: the effect of benzothiadiazole. J. Appl. Sci. Res, 4(12), 2046-2064.

Walters, D., Walsh, D., Newton, A., & Lyon, G. (2005). Induced resistance for plant disease control: maximizing the efficacy of resistance elicitors. Phytopathology, 95(12), 1368-1373.DOI: 10.1094/PHYTO-95-1368.

Gupta, S. K., Gupta, A. K., Sharma, N., & Parasad, D. (2014). Biological Control of Plant Diseases. Approaches and Trends in Plant Disease Management, 53.

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Published

2024-06-28

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How to Cite

Effect of Bio Control Agents on Controlling Root Rot and Damping-off Disease of Cucumber Seedlings Caused by Rhizoctonia solani. (2024). NTU Journal of Agriculture and Veterinary Science, 4(2). https://doi.org/10.56286/1rk5gp32