Evaluation the mechanisms action of plant growth promoting rizobacteria under laboratory condition
DOI:
https://doi.org/10.56286/xhfwyj96Keywords:
indole-3-acetic acid (IAA), phosphate solubilization, rhizobacteria, siderophore,Abstract
This research was conducted to evaluate the efficiency of some mechanisms of rhizobacteria that promote plant growth and to recommend their use as biofertilizers. Therefore, atmospheric nitrogen-fixing bacteria were isolated from the root nodules of six legume species (Vicia faba, Trifolium repens, Lens culinaris, Trigonella foenum-graecum, Lens culinaris subsp orientalis and Medicago sativa). These isolates were positive for most biochemical tests (Urease production, Congo red capture, Bromothymol blue capture, Lactose fermentation and Growth on glucose peptone agar), as well as to test the results of the effect of direct (nitrogenase activity, phosphate solubilization and indol production) and indirect (siderophore production) mechanisms. This indicates their effectiveness in providing the plant with necessary elements. All isolates were able to produce indole acetic acid (IAA) at varying levels. The ability of the legume isolate (MA6) to grow on glucose-peptone agar may indicate that it is not a Rhizobium, unlike the other five isolates. After detection of the 16S rRNA gene sequence of this isolate, it was found with 95% similarity to the global isolate Serratia surfactantfacien strain YD25 registered in GenBank. These results can contribute to recommend the necessity of using these isolates as well as emphasizing the use of the S. surfactantfacien isolate, to promote plant growth as a green and environmentally friendly biofertilizer to sustainably improve agricultural production.
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