Studying of- qualitative-properties-of- gelatin-extracted from- bovine-hides as-by-product and-marshmallow made from it

Authors

  • mohammed saab Mosul University
  • Basmaa Saaduldeen Sheet

DOI:

https://doi.org/10.56286/b68t9h17

Keywords:

gelatin, bovine hide, By-product, Extraction, Marshmallow

Abstract

Leaving animal tissue waste in the form of waste after slaughter pollutes the environment, so one of the goals of this study is to try to use it to make gelatin. Other goals include examining the functional, physical, and chemical properties of gelatin, comparing them to commercial gelatin, which has practical and economic value and researching some of the characteristics of marshmallows made from it. The findings indicated that the viscosity, in comparison to commercial gelatin, which reached 4.1°cP, hide gelatin's gel strength, elasticity, cohesiveness, melting point, and gelation dropped to 3.5 cP, 271.2 g, 5.00 mm, 0.90, 29.00, and 14.57°C, respectively. cP, 446.6 g, 5.07 mm, 0.99, 32.67, and 17.67 °C, the pH values of skin gelatin were higher, at 5.60 and 4.50, respectively. After 30 and 60 minutes, the gelatin's hide expanded and stabilized the foam more than the commercial gelatin (75.25, 90.96, and 84.75%, respectively) (63.37, 90.91, and 87.88%, respectively).This study discovered 18 distinct types of amino acids, including threonine, tyrosine, asparagine, aspartic acid, leucine, phenylalanine, serine, glycine, histidine, lysine, alanine, arginine, cysteine, valine, isoleucine, methionine, proline and hydroxyproline. The gelatin's hide had lower levels of these amino acids than the commercial gelatin. In contrast to the commercial marshmallows, which achieved the same qualities of 589.5 g, 4.7 mm, and 0.87, the factory-made marshmallows' gel strength, elasticity, and cohesiveness were poor, measuring 465.8 g, 4.4 mm, and 0.87, respectively.  The marshmallow sample prepared from commercial gelatin T0 received the highest marks for all qualities in the sensory evaluation, followed by the marshmallow sample made from gelatin from cow hides (T1).This means that it obtained great pleasure scores that were almost similar to the marshmallows made from commercial gelatin; this is due to the properties of the hide gelatin that are similar to those of commercial gelatin. Therefore, gelatin's hide, as an inexpensive by-product that can be used as a stabilizer, thickener and foaming component in many foods, as well as other applications.

References

Usman, M., Ishaq, A., Regenstein, J. M., Sahar, A., Aadil, R. M., Sameen, A., ... & Alam, A. (2023). Valorization of animal by-products for gelatin extraction using conventional and green technologies: A comprehensive review. Biomass Conversion and Biorefinery, 1-13.

Xu, M., Wei, L., Xiao, Y., Bi, H., & Yang, H. (2017). Physicochemical and function- al properties of gelatin extracted from Yak skin. International Journal of Biological Macromolecules, 95, 1246–1253.

Amertaningtya, D., Bachrudin, Z. J., Chin, K. B., & Erwanto, Y. (2019). Characteristics of gelatin extracted from Indonesian local cattle hides using acid and base curing. Pakistan Journal of Nutrition, 18(5), 443–454.

Lv, L. C., Huang, Q. Y., Ding, W., Xiao, X. H., Zhang, H. Y., & Xiong, L. X. (2019). Fish gelatin: The novel potential applications. Journal of Functional Foods, 63, 103581.83.

Sultana, S., Ali, M. E., & Ahamad, M. N. U. (2018). Gelatine, collagen, and single cell proteins as a natural and newly emerging food ingredients. In Preparation and processing of religious and cultural foods (pp. 215-239). Woodhead Publishing.

Hanani, Z. N., Roos, Y. H., & Kerry, J. P. (2014). Use and application of gelatin as potential biodegradable packaging materials for food products. International journal of biological macromolecules, 71, 94-102.

Niu, S., Wang, S., Lin, L., Liu, Y., Zhou, Y. S., Hu, Y., & Wang, Z. L. (2013). Theoretical study of contact-mode triboelectric nanogenerators as an effective power source. Energy & Environmental Science, 6(12), 3576-3583.

Alfaro, A. T., Biluca, F. C., Marquetti, C., Tonial, I. B., & de Souza, N. E. (2014). African catfish (Clarias gariepinus) skin gelatin: Extraction optimization and physical–chemical properties. Food research international, 65, 416-422.

Fatimah, S., Sarto, S., Fahrurrozi, M., & Budhijanto, B. (2023). Characterization and development of gelatin from cow bones: Investigation of the effect of solvents used for soaking beef bones. Applied sciences, 13(3), 1550.

Vázquez-Ortiz, F. A., Caire, G., Higuera-Ciapara, I., & Hernández, G. (1995). High performance liquid chromatographic determination of free amino acids in shrimp. Journal of Liquid Chromatography & Related Technologies, 18(10), 2059-2068.

Roy, B. C., Das, C., Hong, H., Betti, M., & Bruce, H. L. (2017). Extraction and characterization of gelatin from bovine heart. Food Bioscience, 20, 116-124.ocellatum (Dinophyceae). Front. Vet. Sci., 7(1) 83.

Wainewright, F. W. (1977) Physical tests for gelatin products. In: The Science Technology of Gelatins. (Ward, A.G. and Courts, A., Eds), Academic Press Inc., London: 508-531.

Ratnasari, I. (2016). Physico-chemical characterization and skin gelatin rheology of four freshwater fish as alternative gelatin source. Aquaculture, Aquarium, Conservation & Legislation, 9(6), 1196-1207.

Cho, S. M., Kwak, K. S., Park, D. C., Gu, Y. S., Ji, C. I., Jang, D. H., ... & Kim, S. (2004). Processing optimization and functional properties of gelatin from shark (Isurus oxyrinchus) cartilage. Food hydrocolloids, 18(4), 573-579.

Stone, H., Bleibaum, R. N., & Thomas, H. A. (2020). Sensory evaluation practices. Academic press

Al-Rawi, Khasha Mahmoud and Khalaf Allah, Abdul Aziz Muhammad (1980). Developing Agricultural Agriculture, Mosul University Press.

Samatra, M. Y., Razali, U. H. M., Shaarani, S. M., Roslan, J., Ramli, R. A., & Noor, N. Q. I. M. (2024). Physicochemical and Functional Properties of Buffalo (Bubalus bubalis) Bone Gelatin Extracted Using Acid Pre-Treatment. Future Foods, 100428.

GMIA (2019).Gelatin Manufacturers Institute of Amerca America , pp.1-26.

Ahmad, T., Ismail, A., Ahmad, S. A., Khalil, K. A., Teik Kee, L., Awad, E. A., & Sazili, A. Q. (2019). Physicochemical characteristics and molecular structures of gelatin extracted from bovine skin: effects of actinidin and papain enzymes pretreatment. International Journal of Food Properties, 22(1), 138-153.

Al-Hassan, A. A. (2020). Gelatin from camel skins: Extraction and characterizations. Food Hydrocolloids, 101, 105457.

Amiza, M. A., Wan Maizatul Shima, W. M., Nor Hayati, I., & Nizaha Juhaida, M. (2015). Optimization of gelatin extraction conditions from Cobia (Rachycentron canadum) skin and its physicochemical characteristics as compared to bovine gelatin. International Food Research Journal, 22(1).

Kuan, Y. H., Nafchi, A. M., Huda, N., Ariffin, F., & Karim, A. A. (2016). Comparison of physicochemical and functional properties of duck feet and bovine gelatins. Journal of the Science of Food and Agriculture, 97(5), 1663–1671.

Sarbon, N. M., Badii, F., & Howell, N. K. (2013). Preparation and characterisation of chicken skin gelatin as an alternative to mammalian gelatin. Food hydrocolloids, 30(1), 143-151.

Abedinia, A., Mohammadi, A., & Sharifi, M. (2020). Poultry gelatin: Characteristics, developments, challenges, and future outlooks as a sustainable alternative for mammalian gelatin. Trends in Food Science & Technology, 104, 14–26.

Liu, H. Y., Han, J., & Guo, S. D. (2009). Characteristics of the gelatin extracted from Channel Catfish (Ictalurus Punctatus) head bones. LWT-Food Science and Technology, 42(2), 540-544.

Lau, M. H., Tang, J., & Paulson, A. T. (2000). Texture profile and turbidity of gellan/gelatin mixed gels. Food Research International, 33(8), 665-671.

Chandra, M. V., & Shamasundar, B. A. (2015). Texture profile analysis and functional properties of gelatin from the skin of three species of fresh water fish. International Journal of Food Properties, 18(3), 572-584.

Mohammadnezhad, S., & Farmani, J. (2022). Rheological and functional characterization of gelatin and fat extracted from chicken skin for application in food technology. Food Science & Nutrition, 10(6), 1908-1920.

Gudipati, V. (2013). Fish gelatin: A versatile ingredient for the food and pharmaceutical industries. Marine Proteins and Peptides: Biological Activities and Applications, 271-295.

Mad-Ali, S., Benjakul, S., Prodpran, T., & Maqsood, S. (2016). Characteristics and gel properties of gelatin from goat skin as influenced by alkaline-pretreatment conditions. Asian-Australasian journal of animal sciences, 29(6), 845.

Jridi, M., Nasri, R., Lassoued, I., Souissi, N., Mbarek, A., Barkia, A., Barkia, A. & Nasri, M. (2013). Chemical and biophysical properties of gelatins extracted from alkalipretreated skin of cuttlefish (Sepia officinalis) using pepsin. Food Research International, 54, 1680-1687.

Damodaran S. (1997). Protein-stabilized foams and emulsions. Damodaran S and Paraf A editors. Food proteins and their applications. 1st ed. New York:Marcel Dekker. p 57-110.

Jamaluddin, J., Abbasiliasi, S., Sazili, A. Q., & Mustafa, S. (2024). Differentiation of Extracted Gelatins from Porcine, Bovine and Chicken Origins Based on their Physical, Chemical and Structural Properties using Analytical Techniques. Journal of Biochemistry, Microbiology and Biotechnology, 12(1), 17-26.

Rasli, H. I., & Sarbon, N. M. (2015). Effects of different drying methods on the rheological, functional and structural properties of chicken skin gelatin compared to bovine gelatin. International Food Research Journal, 22(2), 584.

Santoso, U., Pranoto, Y., Afriyanti, Y. T., & Mulyani, S. (2019). The physical and chemical properties of marshmallow made from Bufallo (Bubalusbubalis) hide gelatin compared to commercial gelatin. Journal of Applied Food Technology, 6(2).

Mulyani, S., Bintoro, V. P., Legowo, A. M., & Santoso, U. (2019).The Emulsifying and Foaming Properties of Buffalo (Bubalus bubalis) Hide Gelatin Extracted Using Alkali Acid Compared to Commercial Gelatin. ISSN: 2319-7064,p.543-546.

Hafidz, R. M. R. N., Yaakob, C. M., Amin, I., & Noorfaizan, A. (2011). Chemical and functional properties of bovine and porcine skin gelatin. International Food Research Journal, 18(2), 787-791.

Suptijah, P., S.H. Suseno & C. Anwar. 2013. Analisis kekuatan gel (gel strength) produk permen jelly dari gelatin kulit ikan cucut dengan penambahan karaginan dan rumput laut. Jurnal Pengolahan Hasil Perikanan Indonesia. 16 (2): 183-191.

Ahmad, T., Ismail, A., Ahmad, S. A., Khalil, K. A., Kee, L. T., Awad, E. A., & Sazili, A. Q. (2020). Extraction, characterization and molecular structure of bovine skin gelatin extracted with plant enzymes bromelain and zingibain. Journal of food science and technology, 57, 3772-3781.?

Aziza, I. N., Darmanto, Y. S., & Kurniasih, R. A. (2019). The Effect of Gelatin from Different Fish Skin on Physical and SensoryCharacteristics of Marsmallow. Jurnal Perikanan Universitas Gadjah Mada, 21(1), 17-23.

Rather, J. A., Akhter, N., Ashraf, Q. S., Mir, S. A., Makroo, H. A., Majid, D., ... & Dar, B. N. (2022). A comprehensive review on gelatin: Understanding impact of the sources, extraction methods, and modifications on potential packaging applications. Food Packaging and Shelf Life, 34, 100945.

Gudmundsson, M. (2002). Rheological properties of fish gelatins. Journal of Food Science, 67(6), 2172-2176.?

Caecar, L. M., Junianto, J., Haetami, K., & Rostini, I. (2019). The effect of Osteochilus hasselti (Valenciennes, 1842) bone gelatin addition toward jelly candy’s preferences level. World Scientific News, 127(3), 139-152.?

Additional Files

Published

2025-09-28

Issue

Section

Articles

How to Cite

Studying of- qualitative-properties-of- gelatin-extracted from- bovine-hides as-by-product and-marshmallow made from it. (2025). NTU Journal of Agriculture and Veterinary Science, 5(3). https://doi.org/10.56286/b68t9h17

Similar Articles

1-10 of 23

You may also start an advanced similarity search for this article.