Comparison of the Effects of Different Levels of L-Carnitine Supplementation on Growth and Egg Production in Three local Quail Lines Using In Ovo Injection and Dietary Feeding Methods

Authors

  • lajan ahmed College of Agricultural Engineering Sciences, Salahaddin Uiniversity- Erbil https://orcid.org/0000-0002-3696-1108
  • Gulstan Hamadameen Radha Department of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil Dept. of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil Dept. of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil https://orcid.org/0009-0007-0630-3853
  • Aihan Jalal Khalil Department of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil  
  • Parizan Wshyar Ibrahim   Department of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil      

DOI:

https://doi.org/10.56286/0zs5hb64

Keywords:

L- Carnitine, Ovo Feeding, Diets Supplementation, Growth performance, Quails

Abstract

Two studies were conducted to examine the impact of in-ovo injection and dietary addition of L-carnitine (LC) on the growth and egg laying traits of three lines of local quails: desert, brown, and white. The first experiment involved 480 fertile quail eggs were utilized, on the 14th day of incubation, live embryos inside the eggs were injected in the amniotic fluid with varying doses of L-carnitine (0, 4, 8, and 12 mg dissolved in 100 L of a commercial diluent). In the second experiment, 480 one-day-old, unsexed quail chicks were divided into four experimental treatments included: The first group was given the basal diet (control), while the other groups were provided with the basal diet supplemented with different levels of L-carnitine (150, 350, and 500 mg/kg). The evaluation of statistics indicated that quail lines, in-ovo injection, and dietary supplementation with L-carnitine significantly (p?0.01) influenced all the studied traits, except for the first egg weight. The interaction between quail line and in-ovo injection treatments was only significant at age of maturity. Conversely, the interaction between the quail line and the dietary supplementation had a significant impact on feed intake, feed conversion ratio, and age at maturity. According to the present results, it can be established that both in ovo injection and dietary supplementation of L-carnitine significantly influenced the productive characteristics traits of quails. In particular, the study revealed that a high in- ovo injection dose of 4 mg/100 µL of diluent and a feed supplement at 350 mg/kg feed resulted in improved production efficiency for quail chicks, except for the initial egg weight, there was no statistically significant association between the feed addition and in-ovo injection treatments.

Author Biographies

  • Gulstan Hamadameen Radha, Department of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil Dept. of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil Dept. of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil

      Department of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil                                                                                                                   

  • Aihan Jalal Khalil, Department of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil  

      Department of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil                                                                                                                   

  • Parizan Wshyar Ibrahim,   Department of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil      

      Department of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University -Erbil                                                                                                                   

References

. Abd El-Azeem, N. A., Abdo, M. S., Madkour, M., and El-Wardany, I. (2014). Physiological and histological responses of broiler chicks to inovo injection with folic acid or L-Carnitine during embryogenesis. Global Veterinary, 13 (4): 544 – 551. https://doi.org/10.5829/idosi.gv.2014.13.04.85231

. Abdel-Fattah, S.A., and Shourrap, M.I. (2012). Physiological effects of in ovo L carnitine and embryonic thermal conditioning on pre and posthatch development of broiler chicks. In 3rd Mediterranean Poultry Summit and 6th International Poultry Conference, 26-29. https://www.researchgate.net/profile/Mohamed-Shourrap/publication/316441817

. Ahmed, L.S. (2022). Impact of egg shell and spots colour on the quality of hatching eggs derived from three lines of local quail. Iraqi Journal of Agricultural Sciences, 53(6): 1256-1269. https://doi.org/10.36103/ijas.v53i6.1640

. Ahmed, L.S., and AL-Barzinji, Y.M.S. (2019). Genetic diversity among local quail using RAPD-DNA marker. Revista Cientifica-Facultad de Ciencias Veterinarias, 29(3): 178-185.

. Ahmed, L.S., and Al-Barzinji, Y.M.S. (2020). Comparative study of hatchability and fertility rate among local quails. Iraqi Journal of Agricultural Science, 51(3): 744-751. https://doi.org/10.36103/ijas.v51i3.1028

. Ahmed, L.S., and Al-Barzinji, Y.M.S. (2020). Three candidate genes and its association with quantitative variation of egg production traites of local quail by using pcr-rflp. Iraqi Journal of Agricultural Science, 51,124-131. https://doi.org/10.36103/ijas.v51iSpecial.889

. Ahmed, L.S., and Al-Barzinji, Y.M.S. (2022). Detection of quantitative loci correlation with growth traites in local quail using PCR-RFLP technique. Iraqi Journal of Agricultural Sciences, 53(1):16-26. https://doi.org/10.36103/ijas.v53i1.1501

. AL-Jomaily, T.K., and Taha, A.T. (2020). Effects in-ovo injection of L-carnitine on hatchability, productive and physiological traits of Japanese quail (Coturnix Japonica). Plant Archives, 20(2): 4051-4055. https://doi.org/ 10.4314/sajas.v42i2.6

. Al-Neemy, M.A.S., Abd Al-Bake, T.M., and Ahmed, F.T. (2014). Effect of genetic group and added fat source in grower ration on some reproductive traits quail. Tikrit Journal for Agricultural Sciences, 14(2): 283- 294 http://www.plantarchives.org/SPECIAL%20ISSUE%2021-1/19_

. Arslan, C. (2006). L-carnitine and its use as a feed additive in poultry feeding a review. Revue de médecine vétérinaire, 157(3): 134-142. . https://doi.org/10.1017/S0043933911000304

. Baumgartner, M., and Blum, R. (1993). L-carnitine in animal nutrition. vitamine und weitere zusatzstoffe bei mensch und tier (vitamins and other supplements for humans and animals).(G 5. Flachowsky and R Schubert, editors). Friedrich-Schiller Universitaet, Jena, Germany, 413-418. https://www.example.edu/paper.pdf

. Bremer, J. (1983). Carnitine--metabolism and functions. Physiological reviews, 63(4): 1420-1480.

. Burtle, G. J., and Liu, Q. (1994). Dietary carnitine and lysine affect channel catfish lipid and protein composition. Journal of the World Aquaculture Society, 25(2): 169-174. https://doi.org/10.1111/j.1749-7345.1994.tb00178.x

. Cave, M. C., Hurt, R. T., Frazier, T. H., Matheson, P. J., Garrison, R. N., McClain, C. J., and McClave, S. A. (2008). Obesity, inflammation, and the potential application of pharmaconutrition. Nutrition in Clinical Practice, 23(1): 16-34. https://doi.org/10.1177/011542650802300116

. Corduk, M., and Sarica, S. (2008). Effects of L-carnitine in layer diets containing different fat sources and energy levels on hen performance and egg quality. South African Journal of Animal Science, 38(3): 260-270. . https://hdl.handle.net/10520/EJC94572

. Co?kun, I., Cayan H., Yilmaz O., Taskin A., Tahtabiçen E., and Samli H. (2014). Effects of in ovo pollen extract injection to fertile broiler eggs on hatchability and subsequent chick weight. Türk Tar?m ve Do?a Bilimleri Dergisi, 1(4): 485-489. https://dergipark.org.tr/en/pub/turkjans/issue/13308/160752.

. Coulter, D. L. (1995). Carnitine deficiency in epilepsy: risk factors and treatment. Journal of Child Neurology, 10(2): 2S32-2S39. https://doi.org/10.1177/0883073895010002S05

. Dawood, I.R., Alneemy, M.A., and Hameed, S.M., (2021). Study of the effect of the genetic group, egg weight, and their interaction on the productive performance of quail birds during the growth stage. Plant Archives, 21(1): 98-103

. Dooley, M., Peebles, E. D., Zhai, W., Mejia, L., Zumwalt, C. D., and Corzo, A. (2011). Effects of L-carnitine via in ovo injection with or without L-carnitine feed supplementation on broiler hatchability and posthatch performance. Journal of Applied Poultry Research, 20(4): 491-497. . https://doi.org/10.3382/japr.2010-00280

. Duncan, D.B. (1955). Multiple ranges and multiple F test. Biometrics, 11, 1-42.

. Foye, O. T., Uni, Z., and Ferket, P. R. (2006). Effect of in ovo feeding egg white protein, ?-hydroxy-?-methylbutyrate, and carbohydrates on glycogen status and neonatal growth of turkeys. Poultry Science, 85(7): 1185-1192. https://doi.org/10.1093/ps/85.7.1185

. Ghonim, A. I. (2021). An attempt to improve hatchabilty percentage of bronze turkey eggs by technique of in ovo injection with l-carnitine. Egyptian Poultry Science Journal, 41(1): 113-127. https://journals.ekb.eg/article_160059.html

. Hrn?ár, C., Verguliaková, S., Svorad, P., Weis, J., Aprášová, H., Mindek, S., Fik, M. and Bujko, J. (2015). Effect of L-carnitine supplementation on fattening and carcass parameters of broiler chickens. Acta fytotechnica et zootechnica, 18(1):15-19. https://www.academia.edu/download/74378301/131-933-1-PB.pdf

. Ibrahim, P.W., Ahmed, L.S., Mahdi, L.S. and Khalil, A.J. (2023). Influence of supplementation of dietary with varying levels of l-carnitine on laying performance and egg quality parameters in different lines of Kurdish quails. Journal of Duhok University, 26(2):72-83.

. Inci, H., Sogut, B., Sengul, T., Sengul, A. Y., and Taysi, M. R. (2015). Comparison of fattening performance, carcass characteristics, and egg quality characteristics of Japanese quails with different feather colors. Revista Brasileira de Zootecnia, 44(11): 390-396. https://doi.org/10.1590/S1806-92902015001100003

. Kazemi-Fard, M., Yousefi, S., Dirandeh, E., and Rezaei, M. (2015). Effect of different levels of L-carnitine on the productive performance, egg quality, blood parameters and egg yolk cholesterol in laying hens. Poultry Science Journal, 3(2): 105-111.

. Kermanshahi, H., Golian, A., Khodambashi Emami, N., Daneshmand, A., Ghofrani Tabari, D., and Ibrahim, S. A. (2017). Effects of in ovo injection of threonine on hatchability, intestinal morphology, and somatic attributes in Japanese quail (Coturnix japonica). Journal of applied animal research, 45(1): 437-441. https://doi.org/10.1080/09712119.2016.1206902

. Mahdi, L.S., Ahmed, L.S., Ibrahim, P.W., and Hamadameen, I.G. (2023). Efficiency of different levels of l-carnitine supplementation on growth performance and blood parameters in three lines of kurdish quails (coturnix coturnix). Iraqi Journal of Agricultural Sciences, 54(6): 1509- 1519.

. Mc Dowell, L.R. (1989). Vitamins in Animal Nutrition. Ed. Tony J. Cunha, Chapter 16: 393-394, Academic Press, Inc., 1250 Sixth Avenue, San Diego, California 92101. http://www.ucv.ve/fileadmin/user_upload/facultad_agronomia/Producion_Animal/Vitamins_in_Animal_and_Human_Nutrition.pdf

. Nouboukpo, K.E., Tana, K., Kamers. B., Everaert. N. and Willemsen, H. (2010) Effect of In vivo administration of L-carnitine on hatching events and juvenile performance of layer type chicken. International Journal of Poultry Sciences. 9(10), 980-983. https://cir.nii.ac.jp/crid/1130000795354822400

. NRC. (1994). Nutrient Requirements of Domestic Animals. Nutrient Requirements of Poultry (9th ed). National Academy Press, Washington, D.C., USA

. Petek, M., Çavu?oglu, E., Odaba?i, F., and Ye?ilba?, D. (2022). Comparison of egg quality characteristics of different quail lines. Journal of Research in Veterinary Medicine, 41(1): 27-31. https://dergipark.org.tr/en/pub/jrvm/issue/71435/1025604

. Rabie, M. H., Ismail, F. S. A., and Ahmed, A. A. S. (2015). Effect of in ovo injection of L-carnitine at different incubational ages on egg hatchability in broiler breeders and post-hatch performance. Asian Journal of Animal and Veterinary Advances, 10 (12): 875-884. https://scialert.net/abstract/?doi=ajava.2015.875.884

. Rebouche, C.J. (1992). Carnitine function and requirements during the life cycle. The FASEB Journal, 6(15): 3379- 3386. . https://doi.org/10.1096/fasebj.6.15.1464372

. Rizk, Y.S., Beshara, M.M., Hanan, S.M., and Hassan, A.L. (2022). Impact of supplementing l-carnitine on productive and reproductive performance of mamoura chicken during the winter season. Egyptian Poultry Science Journal, 42 (2): 171-185. https://epsj.journals.ekb.eg/article_249544.html

. Salmanzadeh, M., Ebrahimnezhad, Y. and Shahryar, H.A. (2013).The effects of in-ovo injection of L-carnitine on hatching traits, growth performance, and carcass characteristics of turkey poults. Bulletin of Environment, Pharmacology and Life Sciences, 2(11): 125-128. https://epsj.journals.ekb.eg/article_249544.html

. Salmanzadeh, M., Ebrahimnezhad, Y., and Shahryar, H. A. (2012). Effects of in ovo administration of L-carnitine on hatchability, subsequent performance, carcass traits and blood cholesterol of turkey poults. Revue de Médecine Vétérinaire, 163(10): 448-453.

. Sarica, S., Corduk, M., Ensoy, U., Basmacioglu, H., and Karatas, U. (2007). Effects of dietary supplementation of L-carnitine on performance, carcass and meat characteristics of quails. South African Journal of Animal Science, 37(3): 189-201. https://hdl.handle.net/10520/EJC94534

. Sato, M., Tachibana, T., and Furuse, M. (2006). Heat production and lipid metabolism in broiler and layer chickens during embryonic development. Comparative Biochemistry and Physiology Part A. Molecular and Integrative Physiology, 143(3): 382-388. https://DOI: 10.1016/j.cbpa.2005.12.019

. Sherif, S.K. (2021). Effect of in-ovo injection of biotin, and carnitine on hatching and growth performance in broiler chicks. Egyptian Poultry Science Journal, 41(2): 333-350. https://epsj.journals.ekb.eg/article_182511.html

. ur Rehman, A., Rafi, U., Yasmeen, R., and Fayyaz, M. (2023). Effects of nutritional supplement E-Sel on the Egg production, Fertility, and Hatchability of Indian peafowl. Journal of Wildlife and Biodiversity, 7(4): 90-100. https://doi.org/10.5281/zenodo.7043125

. Waller, A. (2007). Take a fresh look at broiler nutrition-Feed accounts for most of the cost of broiler production but the most economic option is not always to reduce feed cost. The impact of the current rise in. Poultry International, 46(9): 12-15.

. Yair, R., Shahar, R., and Uni, Z. (2013). Prenatal nutritional manipulation by in ovo enrichment influences bone structure, composition, and mechanical properties. Journal of animal science, 91(6): 2784-2793. https://doi.org/10.2527/jas.2012-5548

. Yalçin, S., Ergün, A., Erol, H., Yalcin, S., and Özsoy, B. (2005). Use of L-carnitine and humate in laying quail diets. Acta Veterinaria Hungarica, 53 (3): 361-370. https://doi.org/10.1556/avet.53.2005.3.9

. Zeyner, A., and Harmeyer, J. (1999). Metabolic functions of L?Carnitine and its effects as feed additive in horses. A review. Archives of Animal Nutrition, 52(2): 115-138. https://doi.org/10.1080/17450399909386157

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Published

2025-03-27

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How to Cite

Comparison of the Effects of Different Levels of L-Carnitine Supplementation on Growth and Egg Production in Three local Quail Lines Using In Ovo Injection and Dietary Feeding Methods. (2025). NTU Journal of Agriculture and Veterinary Science, 5(1). https://doi.org/10.56286/0zs5hb64

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