Reducing Corn Ear Rot Disease and Fumonisin Content Through Biological and Chemical Treatments
DOI:
https://doi.org/10.56286/f52x0261Keywords:
Fusarium verticillioides Fusarium proliferatum stalk rot ear rot Corn Bacillus subtilis Urea BionAbstract
Isolation results from corn seeds revealed the presence of two fungal species, F. verticillioides and F. proliferatum.The study demonstrated that treatment with B. subtilis, Bion, and urea was highly effective in inhibiting the mycelia growth of both fungi.The highest rates of growth inhibition were recorded for F. proliferatum at 70.21% and for F. verticillioides at 62.45% when using B. subtilis.The results showed that the highest average infection severity of corn cob rot was recorded with F. verticillioides at 0.51, with a significant difference from F. proliferatum at 0.46.The local variety was the most susceptible to infection, with an infection severity of 0.52, while the hybrid 7776 showed the lowest severity at 0.45. The lowest average infection severity was recorded in the urea treatment, amounting to 0.38, with a significant difference from the control treatment at 0.62.The results also indicated that the highest average amount of fumonisins was recorded in F. verticillioides treatments, at 7.79 mg/kg seed, with a significant difference from F. proliferatum at 7.22 mg.kg-1 seed.Conversely, the lowest average amount of fumonisins was recorded in the urea treatment, at 5.39 mg.kg-1 seed, with a significant difference from the control treatment.
References
Abbasian, A. (2012). Background Paper for the Competitive Commercial Agriculture in Sub–Saharan Africa (CCAA) Study. Maize; International Market Profile. Food and Agriculture Organization of the United Nations (FAO).
Al-Numan, Doaa Abdel-Nafi Abdel-Qader Hamo (2021) The effect of treatment with the fungus Trichoderma harzianum and the chemical inducer BION on the biochemical response of genotypes of chickpea plants infected with root rot. Master Thesis . Plant Protection Department. College of Agriculture and Forestry. University of Al Mosul.
Al-Toufan, Nour Kazem Nasser (2020) Isolating and identifying the fungi that cause stem rot and Fusarium shoot disease in yellow corn and evaluating the effectiveness of some varieties and biological agents in combating them and preventing them from producing the mycotoxin Fumonisin. Master's thesis, College of Agriculture, University of Karbala, Iraq.
Baard, V., Bakare, O. O., Daniel, A. I., Nkomo, M., Gokul, A., Keyster, M., & Klein, A. (2023). Biocontrol potential of Bacillus subtilis and Bacillus tequilensis against four Fusarium species. Pathogens, 12(2), 254.?
Barnett, H. L. , and B.B. Hunter, Illustrated Genera of Imperfecti Fungi, 3rd ed. , Bungress publishing company , Minneapolis, Minnesota . pp. 1972 .
C.O.R.N . newsletter // 2016-32 // corn ear rots: identification, quantification, and testing for mycotoxins
Cavaglieri, L., Orlando, J. R. M. I., Rodriguez, M. I., Chulze, S., & Etcheverry, M. (2005). Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize root level. Research in microbiology, 156(5-6), 748-754.
Chaopei D., Yabin W., Jingyang G., Zijian Z., Cong M., Peipei M., Jiafa C., and Jianyu W., (2018). Field Inoculation and Classification of Maize Ear Rot Caused by Fusarium verticillioides, Bio-protocol 8 (23): e3099. DOI: 10.21769/BioProtoc.3099.
Colson-Hanks, E.S., S.J., Allen and B.J. Deverall. 2000. Effect of 2,6- dichloroisonicotinic acid or benzothiadiazole on Alternaria leaf spot, bacterial blight and Verticillium wilt in cotton under field conditions. Australas. Plant Pathol., 29: 170-177.
Desjardins, A. E. (2006). Fusarium Mycotoxins: Chemistry, Genetics, and Biology. St. Paul, Minnesota, USA: The American Phytopathological Society.
Dong, C., Wu, Y., Gao, J., Zhou, Z., Mu, C., Ma, P., ... & Wu, J. (2018). Field inoculation and classification of maize ear rot caused by Fusarium verticillioides. Bio-protocol, 8(23), e3099-e3099
Einloft, T. C., Hartke, S., de Oliveira, P. B., Saraiva, P. S., & Dionello, R. G. (2021). Selection of rhizobacteria for biocontrol of Fusarium verticillioides on non-rhizospheric soil and maize seedlings roots. European Journal of Plant Pathology, 160(3), 503-518.?
Ekwomadu, T.I., Akinola, S.A. and Mwanza, M., 2021. Fusarium mycotoxins, their metabolites (Free, emerging, and masked), food safety concerns, and health impacts. International Journal of Environmental Research and Public Health, 18(22), p.11741.
Fallahi, M., Saremi, H., Javan-Nikkhah, M., Somma, S., Haidukowski, M., Logrieco, A. F., & Moretti, A. (2019). Isolation, molecular identification and mycotoxin profile of Fusarium species isolated from maize kernels in Iran. Toxins, 11(5):297.
FAO(2023).FAOStat.FAO.Rome.Http://www.fao.org/faostat
Farhat G.A.A., Salama N.H.H., El-Bana M.A., Arafa S.G., (2018) EFFECT OF FOLIAR APPLICATION OF SOME NUTRIENTS ON MAIZE EARS ROT DISEASE, SYNTHASE OF ANTI-DEFENSE COMPOUNDS, QUALITY OF MAIZE GRAINS AND BREAD. Zagazig Journal of Agricultural Research. Article 39, Volume 45, Issue 6, November 2018, Page 2389-2408.
Guimarães, R. A., Pherez-Perrony, P. E., Müller, H., Berg, G., Medeiros, F. H. V., & Cernava, T. (2020). Microbiome-guided evaluation of Bacillus subtilis BIOUFLA2 application to reduce mycotoxins in maize kernels. Biological Control, 150, 104370.
Guimarães, R. A., Zanotto, E., Perrony, P. E. P., Zanotto, L. A. S., da Silva, L. J., Machado, J. D. C., ... & de Medeiros, F. H. V. (2021). Integrating a chemical fungicide and Bacillus subtilis BIOUFLA2 ensures leaf protection and reduces ear rot (Fusarium verticillioides) and fumonisin content in maize. Journal of Phytopathology, 169(3), 139-148.?
Guimarães, Rafaela Araújo. How biological and chemical fungicides impact the maize microbiome, Fusarium verticillioides populations and fumonisins content / Rafaela Araújo Guimarães. - 2018. 100 p. : il. Universidade Federal De Lavras.
Jabbar, Abeer Abdel Zahra, Sabah Latif Alwan, Zaidan Khalif Omran (2016) The effect of some chemical induction agents on reducing the infection of yellow maize plants with the fungus Fusarium verticillioides. Kufa Journal of Agricultural Sciences 8 (3): 179 – 199.
Jabbar, Abeer Abdel Zahra, Sabah Latif Alwan, Zidan Khalif Omran (2016) Detection of the fungus Fusarium verticillioides in yellow corn grains and testing the effectiveness of some chemical induction agents in reducing the infection. Kufa Journal of Agricultural Sciences 8 (3): 200 – 220.
Leslie, J. F. and Summerell, B. A. (2006). The Fusarium laboratory manual. Blackwell Publishing. USA. 388p.
Mohammed, S. W., Nayyef, H. J., Sameer, F. O., & Hanoon, A. Y. (2021). Enzyme linked Immunosorbent Assay for Fumonisin B1 detection in local corn seeds from Baghdad-Iraq. Iraqi Journal of Science, 4621-4627.?
Nelson, K.A. and C. Meinhardt (2011). Corn yield response to foliar boron and headline fungicide pp 35.Field day report. Greenley Memorial Res. Cent. Univ. Missouri.
Nelson, P.E.; Toussoun, T.A. and Marasas, W.F.O. (1983) Fusarium species: Anillustrated manual for identification. The Pennsylvania State University Press, University Park.
Ojaghian, S., Wang, L., Zhang, J. Z., & Xie, G. L. (2020). Inhibitory effect of Fungastop and Bion against carrot soft rot caused by Sclerotinia sclerotiorum. Phytoparasitica, 48(1), 95–106. https://doi.org/10.1007/s12600-019-00780-9
Peltonen, J., Kittilä, S., Peltonen-Sainio, P., & Karjalainen, R. (1991). Use of foliar-applied urea to inhibit the development of Septoria nodorum in spring wheat. Crop Protection, 10(4), 260-264.?
Qin, P. W., Xu, J., Jiang, Y., Hu, L., Van Der Lee, T., Waalwijk, C., ... & Xu, X. D. (2020). Survey for toxigenic Fusarium species on maize kernels in China. World Mycotoxin Journal, 13(2), 213-224.?
Rush, C. M., & Lyda, S. D. (1982). Effects of anhydrous ammonia on mycelium and sclerotia of Phymatotrichum omnivorum. Phytopathology, 72(8), 1085-1089.
Small, I. M., Flett, B. C., Marasas, W. F. O., McLeod, A., & Viljoen, A. (2012). Use of resistance elicitors to reduce Fusarium ear rot and fumonisin accumulation in maize. Crop Protection, 41, 10-16
Stockmann-Juvalla, H. and Savolainen, K. (2008). A review of the toxic effects and mechanisms of action of fumonisin B1. Human and Experimental Toxicology 27: 799-809.
Summerell, B.A., Salleh, B. and Leslie, J.F. (2003). A utilitarian approach to Fusarium identification. Plant Disease, 87: 117-128.
Suriani, A. Sebayang, H. Mirsam, S. Pakki, M. Azrai, A. Muis, Control of Fusarium verticillioides on Corn with a Combination of Bacillus subtilis TM3 Formulation and Botanical Pesticides, Saudi Journal of Biological Sciences (2021), doi: https://doi.org/10.1016/j.sjbs.2021.07.083
Torrijos, R., de Melo Nazareth, T., Vila-Donat, P., Mañes, J., and Meca, G. (2022). Use of Mustard Extracts Fermented by Lactic Acid Bacteria to Mitigate the Production of Fumonisin B1 and B2 by Fusarium verticillioides in Corn Ears. Toxins, 14(2), 80.
Triki, E., Trabelsi, I., Amri, M., Nefzi, F., Kharrat, M., & Abbes, Z. (2018). Effect of benzothiadiazole and salicylic acid resistance inducers on Orobanche foetida infestation in Vicia faba. Tunisian Journal of Plant Protection, 13(1), 113-125.
Veverka K., Štolcova J., R?žek P. (2007): Sensitivity of fungi to urea, ammonium nitrate and their equimolar solution UAN. Plant Protect. Sci., 43: 157–164.
Yu, C., Liu, X., Zhang, X., Zhang, M., Gu, Y., Ali, Q., ... & Gu, Q. (2021). Mycosubtilin produced by Bacillus subtilis ATCC6633 inhibits growth and mycotoxin biosynthesis of Fusarium graminearum and Fusarium verticillioides. Toxins, 13(11), 791
Yu, C.; Liu, X.; Zhang, X.; Zhang, M.; Gu, Y.; Ali, Q.; Mohamed, M.S.R.; Xu, J.; Shi, J.; Gao, X.; et al. Mycosubtilin Produced by Bacillus subtilis ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of Fusarium graminearum and Fusarium verticillioides. Toxins 2021, 13, 791. https://doi.org/10.3390/toxins13110791.
ZAINUDIN, NUR AIN IZZATI MOHD; HAMZAH, FARAH AQILA; KUSAI, NOR AZIZAH; ZAMBRI, NUR SYUHADA; and SALLEH, SUHAIDA (2017) "Characterization and pathogenicity of Fusarium proliferatum and Fusarium verticillioides, causal agents of Fusarium ear rot of corn," Turkish Journal of Biology: Vol. 41: No. 1, Article 23. https://doi.org/10.3906/biy-1606-25.



