In Vitro Propagation of Two Grapes, Vitis Vinifera L. cvs. Superior And Red Globe
???? ????? ???? Vitis Vinifera L????? ?????
DOI:
https://doi.org/10.56286/257daf03Keywords:
multiplication, plant growth regulators, , Benzyl Adenine, indole-3- butyric acidAbstract
The study aimed to investigate PGR's effect on in vitro grapes Vitis Vinifera L. cvs. Superior and Red globe single nodes propagation. During the surface sterilization stage (10, 20, and 30%), commercial bleach solution was used as a disinfectant agent for (5, 10, and 15 minutes) immersion periods. In the multiplication stage, explants were planted in Murashige and Skoog (MS) medium enriched with (1, 2, 3, and 4 mg L-1) of Benzyl Adenine (BA) concentrations and the control treatment was (BA-free). The rooting stage included cultivating plantlets from the multiplication stage on half-salt strength MS media supplied with (0, 0.5, 1, and 1.5 mg L-1) indole-3- butyric acid (IBA). The results showed that the highest percentage of uncontaminated explants was achieved in 20% of commercial bleach solutions used for a 10-minute immersion period (90 and 80% for Superior and Red Globe cultivars, respectively). The results of the multiplication stage showed that the highest values of newly formed shoots number and leaves number (4.50 and 20.90, respectively, for the Superior) and (4.10 and 18.50, respectively, for the Red Globe) were recorded in media containing 2 mg L-1 BA. The Superior variety showed the highest rooting percentage (90%) and significant root number (3.55) in a medium containing 1 mg L-1 IBA, while the Red Globe variety had the highest percentage (100%), and the root number (3.50) in the medium contained 0.5 mg L-1. The results of this study provide a rapid and effective propagative propagation system for selected grapes.
References
References
Aazami M. A. (2010). Effect of some growth regulators on “in vitro” culture of two Vitis vinifera L. cultivars. Romanian Biotechnological Letters 15:5229-5232. https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=9c90cb478dae9bb2a643d547572cac5208fa168b
Abido, M. A. M., A. S. A. Hassanen and G. A. Rayan (2013). In vitro propagation of grapevine (Vitis vinifera L.) cv. Muscat of Alexandria for conservation of endangerment. Middle-East Journal of Scientific Research. 13: 328-337. http://DOI: 10.5829/idosi.mejsr.2013.13.3.1926
Ahmed, A. M. N., D. A. Ibrahim and S. P. Uzun (2015). In vitro micropropagation of Vitis vinifera L. in Kurdistan region of Iraq Science Journal of University of Zakho, 3(1), 55–66. Retrieved from DOI:10.25271/2015.3.1.278
Al-Aizari, A. A., R. S. Al-Obeed and M. A. H. Mohamed (2020). Improving micropropagation of some grape cultivars via boron, calcium and phosphate. Electronic Journal of Biotechnology, 48, 95–100. https://doi.org/10.1016/j.ejbt.2020.10.001.
Alizadeh, M., S. K. Singh and B. V. Patel (2010). Comparative performance of in vitro multiplication in four grape (Vitis spp.) rootstock genotypes. International Journal of Plant Production, 4(1): 1735-6814. DOI: 10.22069/IJPP.2012.680
Al-Saeedi, I. H. M. (1982). Cultivation and Production of Vineyards. Mosul University, Press. Ministry of Higher Education and Scientific Research. Republic of Iraq. (In Arabic)
Alzubi, H., L. M. Yepes and M. Fuchs (2012). Enhanced micropropagation and establishment of grapevine rootstock genotypes. International Journal of Plant Developmental Biology 6(1):9-14. http://www.globalsciencebooks.info/Online/GSBOnline/images/2012/IJPDB_6(1)/IJPDB_6(1)9-14o.pdf
Batukaev, A., E, Sobralieva and D. Palaeva (2021). Optimization studies of culture media for in-vitro clonal micropropagation of new grape varieties. International research conference on Challenges and Advances in Farming, Food Manufacturing, Agricultural Research and Education, KnE Life Sciences, pages 757–764. DOI: 10.18502/kls.v0i0.9013
Cassells, A.C. (1991). Problems In Tissue Culture: Culture Contamination. In: Debergh, P.C., Zimmerman, R.H. (eds) Micropropagation. Springer, Dordrecht. 1: 31-44. https://doi.org/10.1007/978-94-009-2075-0_3
Colgecen, H., H. N. buyukkartal, and M. C. Toker (2008). In vitro germination and structure of hard seed testa of natural tetraploid Trifolium pratense L. African Journal of Biotechnology. 7(10): 1473-1478. DOI: 10.5897/AJB07.653
Devlin, R. M., Witham, F. H. 1983. Plant Physiology .4th ed. Wadsworth Publishing Company, Belmont California, U.S.A.
Duncan, D.B. (1955). Multiple Range and Multiple. F Tests. Biometrics. 11: 1–42. https://doi.org/10.2307/3001478
Fahmy, F. J. M. (2003). Tissue Culture Book, Scientific Book House for Publishing and Distribution. Egypt. (In Arabic).
Hartmann, H. T., Kester, D. E., Davies, F. T. and Geneve, R. L. (2002). Plant Propagation, Principles and Practices. 7th Edition.: Prentice Hall Upper Saddle River, New Jersey. U. S. A.
Hashemi, S. A. A., B. N. Sathyanarayana and Z. Sharaf (2020). In Vitro propagation of grape (Vitis vinifera L.) cv. Thompson Seedless. Indian Journal of Pure & Applied Biosciences. 8(5), 421-428. DOI: http://dx.doi.org/10.18782/2582-2845.8347
Javalera, M. F. L., R. T. Rojas, M. E. T. Hernández, M. A. Martínez, I. V.,Arispuro, M. A. I. Osuna and M. R. Domínguez,. (2016). Surface disinfection procedure and in vitro regeneration of grapevine (Vitis vinifera L.) axillary buds. Springer Plus. 5: 453. https://doi.org/10.1186/s40064-016-2081-0
Jundiyah, H. (2002). Fruit Tree Physiology. Arab House for Publishing and Distribution, Egybt. (In Arabic).
Kizi, D. N. E., J. S. Turdiqulovich and I. M. H.kizi, (2022). Effect of bap concentration and content of food environment on “in vitro” regeneration of rizamat (Vitis Vinifera L) cultivar. European Journal of Agricultural and Rural Education (EJARE). Vol. 3 No. 2. https://scholarzest.com/index.php/ejare/article/view/1862/1567
Kryukov, L. A., D. I. Vodolazhsky and R. Kamenetsky-Goldstein (2022). Micropropagation of Grapevine and Strawberry from South Russia: Rapid Production and Genetic Uniformity. Agronomy, 12, 308. https:// doi.org/10.3390/agronomy12020308.
Mahmoud, S. N., Al-Ani, N. K. 2016. Effect of different sterilization methods on contamination and viability of nodal segments of Cestrum nocturnum L. International Journal of Research Studies in Biosciences. 4: 4-9. http://dx.doi.org/10.20431/2349-0365.0401002
Melyan, G., A. Sahakyan and A. Harutyunyan (2015). Micropropagation of grapevine (Vitis vinifera L.) seedless cultivar 'Parvana' through lateral bud development. Vitis, 54:253-255. DOI: https://doi.org/10.5073/vitis.2015.54.special-issue.253-255
Mihaljevie, I., D. Krunoslav, T, Vesna, V. Marija, P. Ankica, C. Zlatko, P. Boris and P. Zorica (2013). In vitro sterilization procedures for micropropagation of ‘Obla?Inska’ sour cherry. Journal of Agricultural Sciences. 58: 117-126. https://DOI: 10.2298/JAS1302117M
Moutia, M., and A. Dookun (1999). Evaluation of surface sterilization and hot water treatments on bacterial contaminants in bud culture of sugarcane. Experimental Agriculture, 35 (3), 265-274. doi:10.1017/S001447979900304X
Murashige, T., F. Skoog (1962). A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Reynolds, A.G. (2017). The Grapevine, Viticulture, and Winemaking: A Brief Introduction. In: Grapevine Viruses: Molecular Biology, Diagnostics and Management (pp. 3-29). Springer, Cham. DOI:10.1007/978-3-319-57706-7_1
Robinson T. (1983). The Organic Constituents of Higher Plants: Their Chemistry and Interrelationships (5th ed.). Cordus Press. Retrieved November 13 2023 from http://catalog.hathitrust.org/api/volumes/oclc/9493355.html.
Sammona, O. S. (2022). Micropropagation of grapevine (vitis vinifera l.) cvs. Red globee and Superior. Iraqi Journal of Agricultural Sciences:53(4):833–849. DOI: https://doi.org/10.36103/ijas.v53i4.1596
Sharp, W. R., D. A. Evans, P. V. Ammirato, and Y. yamada, (1984). Handbook of Plant Cell Culture. Vol 2 (Eds.)., Crop Species, Macmillan, New York., pp. 396–434.
Skoog, F., and C. O. Miller (1957). Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symposia of the Society for Experimental Biology, 11, 118–130. PMID: 13486467.
Srivastava, N., B. Kamal, V. Sharma, Y. K. Negi A. K. Dobriyal, S. Gupta1 and V. S. Jadon (2010). Standardization of sterilization protocol for micropropagation of Aconitum heterophyllum-an endangered medicinal herb. Academic. Arena.2:37-42. https://www.sciencepub.net/academia/aa0206/10_2570_aa0206_62_66.pdf
Tawajen, A. M. M. (1987). Decoration Plants. Ministry of Higher Education and Scientific Research, University of Basra - Iraq. (In Arabic).
van Staden J., E. Zazimalova and E. F. George (2007). Plant Growth Regulators II: Cytokinins, their Analogues and Antagonists. In: George E. F., M. A. Hall and G-J. De Klerk, editors. Plant propagation by tissue culture. 3. The Netherlands: Springer; 2008. pp. 205–226. [Google Scholar]
Wasfy, E. (1995). Plant Growth Regulators and Flowering and their Use in Agriculture. Academic library, Cairo-Egypt. (In Arabic).
Yassin, B. T. (2000). Fundamentals of Plant Physiology, Dar Al Sharq Press, Qatar. (In Arabic).
Yildiz, M. (2002). The effect of Sodium hypochlorite solutions on in vitro seedling growth and shoot regeneration of Flax (Unum usitatissimum). The Science of Nature - Naturwissenschaften. 89: 259-261. DOI:10.1007/s00114-002-0310-6
