The Effect of Different Concentrations of Hydrogen Peroxide at a Temperature of 35°C and with Different Incubation Times on the numbers of Bacillus Subtilis in Raw milk
DOI:
https://doi.org/10.56286/ntujavs.v4i1.794Keywords:
Hydrogen peroxide, ?Raw milk?, Spore bacteria?, TemperatureAbstract
This study was conducted to investigate the potential factors effects on hydrogen peroxide bacterial spores killing severity. This study was achieved in Dairy Science and Technology Department , Food Sciences College, Al-Qasim Green University, Babylon, Iraq, during (2022 2023). Itincluded an experiment evaluation of the effect of 0.01, 0.05, and 0.1% concentrations of hydrogen peroxide at temperature of 35 degrees Celsius during an incubation time of 10, 20, 40, and 80 minutes. After that, the number of bacteria was measured by the plate counting method. The treatments were subjected to heat treatmentsat temperatures of 85, 90, and 95 degrees Celsius with and without added Hydrogen Peroxide for 20 minutes. The results showed that 0.1% Hydrogen Peroxide concentration eliminated entirely vegetative Bacillus bacteria and its spores. The results also indicated the concentration of 0.05%.and Heat treatment 90 and 95 °C eliminated bacterial spores to the comparison to control treatment 4.5×410 Cfu.ml-1 raw milk. The results also show a concentration of 0.01%. with heat treatment at 85, 90, and 95°C reduced the number of bacteria, 45×102 , 35×102 Cfu.ml-1 raw milk, and 25×102, respectively, compared to the control treatment 4.5 ×104 Cfu.ml-1 raw milk.
References
Abed Saleh, N. AL-Huda Muhammed., & Al-Tai, A. A. Alwan,(2020). Study the Effect of Some Chemical and Food Additives on the Stability of Milk Towards Different Heat Treatments, Doctoral dissertation College of Food Sciences, Al-Qasim Green University, Babylon, Iraq.
Adly, A. A. (2010). Oxidative stress and disease: an updated review. Res J Immunol, 3(2), 129-45.?
Ajai, A. I., Ochigbo, S. S., & Ndamitso, M. M. (2012). Proximate and mineral compositions of different raw cow’s milks in Minna.?
Al-Bedrani, D. Ibrahim., Al-Tai, A.A. Alwan., & ALKaisy, Q. Hamed, (2012).Effect of Temperature, Light and Preservatives on the Preservation and organoleptic properties of the Locally Produced Garlic Juice." journal of kerbala university.
AL-janabi, Z. R. H., & Al-Bedrani, D. I. (2022). Studying The Effect Of Adding Different Starter Cultures To The Milk On Chemical, Sensory Properties And Yield Of Soft Cheese. NVEO-NATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO, 572–586.
Al-Saadi, J. M. (2014). Effect of Heat Treatment on Whey Proteins Denaturation in the Presence and Absence of Lactose. Journal of Zankoy Sulaimani-Part A, Special Issue, 16, 271-279.?
Block S S. Peroxygen compounds. In: Block S S, editor. Disinfection, sterilization, and preservation. 4th ed. Philadelphia, Pa: Lea & Febiger; 1991. pp. 167–181.
Boateng, M. K., Price, S. L., Huddersman, K. D., & Walsh, S. E. (2011). Antimicrobial activities of hydrogen peroxide and its activation by a novel heterogeneous Fenton’s?like modified PAN catalyst. Journal of applied microbiology, 111(6), 1533-1543.?
Brudzynski, K. (2006). Effect of hydrogen peroxide on antibacterial activities of Canadian honeys. Canadian journal of microbiology, 52(12), 1228-1237.?
Bumbadiya, M., Singh, R., Pradhan, D., Mann, B., & Arora, S. (2017). Screening of different novel preservatives for milk preservation by microbial analysis. Int J Chem Stud, 5(4):673-677.
Cattani, F., Ferreira, C. A. S., & Oliveira, S. D. (2013). The detection of viable vegetative cells of Bacillus sporothermodurans using propidium monoazide with semi-nested PCR. Food microbiology, 34(1), 196-201.
Chapman, K. W., & Boor, K. J. (2001). Acceptance of 2% ultra-pasteurized milk by consumers, 6 to 11 years old. Journal of dairy science, 84(4), 951-954.?
Chavan, R. S., Chavan, S. R., Khedkar, C. D., & Jana, A. H. (2011). UHT milk processing and effect of plasmin activity on shelf life: A review. Comprehensive Reviews in Food Science and Food Safety, 10(5), 251-268.?
Clark, F.E. (1965) Rhizobia. Methods of Soil analysis.chemical and Microbioloical properties. Methods of soil and .1, part 2:1487-1492.
Collin, F. (2019). Chemical basis of reactive oxygen species reactivity and involvement in neurodegenerative diseases. International journal of molecular sciences, 20(10), 2407.?
Esteban, M. D., Huertas, J. P., Fernández, P. S., & Palop, A. (2013). Effect of the medium characteristics and the heating and cooling rates on the nonisothermal heat resistance of Bacillus sporothermodurans IC4 spores. Food microbiology, 34(1), 158-163.?
Feng, L., Peillex-Delphe, C., Lü, C., Wang, D., Giannakis, S., & Pulgarin, C. (2020). Employing bacterial mutations for the elucidation of photo-Fenton disinfection: focus on the intracellular and extracellular inactivation mechanisms induced by UVA and H2O2. Water Research, 182, 116049.?
Feuerstein, O., Moreinos, D., & Steinberg, D. (2006). Synergic antibacterial effect between visible light and hydrogen peroxide on Streptococcus mutans. Journal of Antimicrobial Chemotherapy, 57(5), 872-876.?
Fox, P., Uniacke-lowe, T., Mcsweeney, P. & O’mahony, J. (2015b). Milk proteins, Dairy Chemistry and Biochemistry. Springer.
Jassim, F. H., Mulakhudair, A. R., & Shati, Z. R. K. (2023). Improving Nutritional and Microbiological Properties of Monterey Cheese Using Lactobacillus acidophilus. IOP Conference Series: Earth and Environmental Science, 1158(11), 112023. IOP Publishing.
Jones, I. A., & Joshi, L. T. (2021). Biocide use in the antimicrobial era: a review. Molecules, 26(8), 2276.?
Leggett, M. J., McDonnell, G., Denyer, S. P., Setlow, P., & Maillard, J. Y. (2012). Bacterial spore structures and their protective role in biocide resistance. Journal of applied microbiology, 113(3), 485-498.?
Mansour , M., Rakhisi, Z., & Ahmady, A. Z. (2015). Isolation and identification of Bacillus species from soil and evaluation of their antibacterial properties. Avicenna Journal of Clinical Microbiology and Infection, 2(1).?
Patil, S. M., Ramu, R., Shirahatti, P. S., Prasad, M. N. N., Kumari, V. B. C., Sujay, S., & Tejaswini, M. (2023). Global Food Safety and Quality: A Worldwide Scenario. In Food Microbial and Molecular Biology (pp. 3–34). Apple Academic Press.
Quigley, L., O'Sullivan, O., Stanton, C., Beresford, T. P., Ross, R. P., Fitzgerald, G. F., & Cotter, P. D. (2013). The complex microbiota of raw milk. FEMS microbiology reviews, 37(5), 664-698.?
Rahimi, E., Abdos, F., Momtaz, H., Torki Baghbadorani, Z., & Jalali, M. (2013). Bacillus cereus in infant foods: prevalence study and distribution of enterotoxigenic virulence factors in Isfahan Province, Iran. The Scientific World Journal, 2013.?
Raikos, V. (2010). Effect of heat treatment on milk protein functionality at emulsion interfaces. A review. Food Hydrocolloids, 24(4):259-265.
Rasham, H. D., Mulakhudair, A. R., & Al-Saadi, J. M. S. (2023). Applying of Microcapsulated Probiotics via Calcium and Zinc Salts into Yoghurt and Study its Chemical, Microbiological and Sensory Properties. IOP Conference Series: Earth and Environmental Science, 1225(1), 12050. IOP Publishing.
Sawasn, K. H., & Abd Ali, A. A.-T. (2022). THE STUDY OF THE MANUFACTURE OF BRAIDED CHEESE FROM SKIM BUFFALO MILK BY USING DIFFERENT SOILS, AND ITS CHEMICAL, RHEOLOGICAL AND SENSORY EVALUATION. ANNALS OF FOREST RESEARCH, 65(1), 8574–8583.
Scheldeman, P., Herman, L., Foster, S., & Heyndrickx, M. (2006). Bacillus sporothermodurans and other highly heat?resistant spore formers in milk. Journal of applied microbiology, 101(3), 542-555.?
Setlow, P. (2014). Germination of spores of Bacillus species: what we know and do not know. Journal of bacteriology, 196(7), 1297-1305.?
Setlow, P. (2016). Spore resistance properties. The bacterial spore: from molecules to systems, 201-215.?
Sharma, R., Rajput, Y. S., & Barui, A. K. (2012). Detection of adulterants in milk: laboratory manual. India: NDRI.?
Tschernjawskaja, M. A., & Belowa, A. S. (1990). Antibacterial mechanism of action of hydrogen peroxide preparations. Zeitschrift fur die Gesamte Hygiene und Ihre Grenzgebiete, 36(2), 86-88.?
