Evaluation of the effectiveness of biofertilization with native bacteria in improving the growth and yield of Trifolium alexandrinum under Gypsiferous soil conditions
DOI:
https://doi.org/10.56286/68w0kr91Keywords:
Sinorhizobium meliloti, Pseudomonas azotoformans , Ensifer melilotIAbstract
The excessive use of chemical fertilizers, particularly nitrogen-based ones, has significantly exacerbated environmental problems.The excessive accumulation of these fertilizers in soil and water leads to severe pollution, threatening biodiversity, disrupting ecological balance, and adversely affecting human and animal health over the long term., the world uses advanced agricultural methods, including biofertilizers, and natural sources of plant nutrients. For this reason, a field experiment was conducted at University of Tikrit, Iraq on 2022, to evaluate the effect of a bio-inoculant prepared from local bacterial isolates, namely B1 Ensifer meliloti, B2 Pseudomonas azotoformans, and B5 Sinorhizobium meliloti, , on the growth and yield of Egyptian clover (Trifolium alexandrinum) under different nitrogen levels (0, 40, and 80 kg N ha-1). The experiment demonstrated that seed inoculation with bacteria B1 and B3 significantly positively impacted plant growth compared to uninoculated seeds or those inoculated with pseuodmonas azotoformans. Additionally, increasing the amount of nitrogen fertilizer improved growth, but the best results were obtained when the highest fertilizer level was combined with inoculation using bacteria B3.
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